other:Article Title: A20 deficiency in myeloid cells protects mice from diet-induced obesity and insulin resistance due to increased fatty acid metabolism.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER ACK lysis buffer Thermo Fisher Scientific cat# A1049201 DNase Roche cat# 04 536 282 001 Brewer thioglycollate medium Sigma cat# B-2551 L-glutamin Lonza cat# BE17-605F RPMI 1640 Caisson Labs cat# RPP24-1LT Sodium pyruvate Sigma-Aldrich cat# S-8636 Penicillin-Streptomycin GIBCO cat#15140-122 fetal calf serum Bodinco n/a LPS (E. coli 0111:B4 strain) InvivoGen n/a IFNy Produced in house n/a Ficoll Paque Plus Sigma cat# GE17-1440-02 BODIPY FL C16 Thermo Fisher Scientific cat# D-3821 GM-CSF Produced in house n/a 2-NBDG Thermo Fisher Scientific cat# N13195 BD Horizon Brilliant Stain Buffer BD Biosciences cat# 566349 recombinant mouse M-CSF Produced in house n/a Estradiol Sigma cat# E8875 Fibronectin Sigma n/a Polybrene Sigma cat# TR-1003 G418 ThermoFisher Scientific cat# 10131035 Glycerol Biosolve cat# 7122301 Lecithin Wako chemicals cat# 120-00832 13C labeled ethyl ester palmitic acid Cambridge Isotope Laboratories cat# CLM-3957-0 Liberase TM Roche cat# 5401119001 Critical commercial assays Aurum Total RNA Isolation Mini Kit BioRad cat# 7326820 iScript cDNA synthesis kit Bio-Rad Laboratories cat# 1708890 SensiFAST SYBR No-ROX Kit GeC Biotech cat# CSA-01190 RNeasy Plus Micro Kit QIAGEN cat# 74034 XFe96 cell culture plates Agilent technologies cat# 101085-004 Seahorse XF Long Chain Fatty Acid Oxidation Stress Test Kit Agilent technologies cat# 103672-100 Bioplex mouse TNF assay Biorad cat# 171G5023M Bioplex mouse IL6 assay Biorad cat# 171G5007M Bioplex mouse MCP1 assay Biorad cat# 171G5019M Bioplex mouse RANTES assay Biorad cat# 171G5022M Mouse IL-18 ELISA kit MBL cat# 7625 Free Fatty Acid Assay Kit Abnova cat# KA1667 RayBio Mouse Leptin ELISA kit RayBio Inc cat# ELM-Leptin-1 Triglyceride Colorimetric Assay Kit Cayman Chemical cat# 10010303 Ultra Sensitive Mouse Insulin ELISA kit Crystal Chem cat# 90080 Agilent RNA 600 Nano kit Agilent Technologies n/a SMART-Seq V4 Ultra Low Input kit Takara cat# 634888 NEBNext Ultra DNA Library Prep kit New England Biolabs cat# E7370S Deposited data RNaseq data this paper GEO: GSE182155 Experimental models: Cell lines mouse: primary macrophages (BMDM and peritoneal) this paper n/a (Continued on next page) Cell Reports 36, 109748, September 21, 2021 e2
Article Title: OTULIN Prevents Liver Inflammation and Hepatocellular Carcinoma by Inhibiting FADD- and RIPK1 Kinase-Mediated Hepatocyte Apoptosis.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER mouse: MyD88 / Adachi et al., 1998 n/a mouse: Tnf / Pasparakis et al., 1996 n/a mouse: Tnfr1 / Pfeffer et al., 1993 n/a mouse: Ifnar / M€uller et al., 1994 n/a Oligonucleotides qPCR primers see Table S1 n/a n/a Software and Algorithms ZEN (blue edition) Zeiss https://www.zeiss.com/microscopy/int/products/ microscope-software/zen.html FlowJo.10 n/a https://www.flowjo.com/ GraphPad Prism 8 n/a https://www.graphpad.com/ Fluostar Omega BMG Labtech https://www.bmglabtech.com/fluostar-omega/ Other Axioscan Zeiss n/a BDSymphony BD Biosciences n/a LightCycler 480 II Roche n/a Amersham Imager 600 GE Healthcare n/a
Recombinant:Article Title: OTULIN Prevents Liver Inflammation and Hepatocellular Carcinoma by Inhibiting FADD- and RIPK1 Kinase-Mediated Hepatocyte Apoptosis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER CK19 Lammer lab n/a hoip Damgaard lab n/a hoil-1 Walczak lab n/a Chemicals, Peptides, and Recombinant Proteins TRIzol reagent Thermo Fisher Scientific cat#15596026 mTNF This paper n/a Collagenase A Sigma-Aldrich cat#11088793001 GST-UBAN Ivan Dikic lab n/a DNase Roche cat#04 536 282 001 DMEM GIBCO cat# 41965-062 DMEM F12 GIBCO cat# 31330-038 non essential aminoacids Lonza cat#BE13-114E L-glutamin Lonza cat# BE17-605F bovine collagen 1 Nutacon n/a Sodium pyruvate Sigma-Aldrich cat# S-8636 Penicillin-Streptomycin GIBCO cat#15140-122 fetal calf serum Bodinco n/a Complete, EDTA-free Protease Inhibitor Cocktail Tablets Roche cat#11873580001 PhosStop Roche cat# 04906837001 Antigen retrieval buffer Dako cat# S-203130 hydrogen peroxide 30% Sigma-Aldrich cat# H-1009 Hematoxylin Mayer’s Sigma cat# 51275-500ml Entellan Merck cat# 107961 Neg 50 Kryo-Medium Thermo Fisher Scientific cat# 6506 Direct Red 80 Sigma cat# 365548 Ac-DEVD-MCA PeptaNova cat# 3171-V Critical Commercial Assays Aurum Total RNA Isolation Mini Kit BioRad cat# 7326820 Sensifast cDNA Synthesis Kit Bioline cat#BIO-65054 SensiFAST SYBR No-ROX Kit GeC Biotech cat# CSA-01190 Vectastain ELITE ABC Kit Standard Vector Laboratories cat# PK-6100 ImmPACT DAB Peroxidase (HRP) Substrate Vector Laboratories cat# SK-4105 Western Lightning Plus ECL Perkin Elmer cat# NEL101001EA TUNEL Millipore cat#17-141 Trygliceride colorimetric assay kit Cayman chemical cat#10010303 Deposited Data TCGA database n/a https://www.cancer.gov/about-nci/organization/ ccg/research/structural-genomics/tcga Experimental Models: Cell Lines mouse: primary hepatocytes this paper n/a OTULINKO MEFs this paper n/a Experimental Models: Organisms/Strains mouse: OTULINFL this paper n/a mouse: Alfp Cre Kellendonk et al., 2000 n/a mouse: MlklFL Murphy et al., 2013 n/a mouse: FaddFL Mc Guire al., 2010 n/a mouse: Ripk1D138N Polykratis et al., 2014 n/a (Continued on next page) Cell Reports 30, 2237–2247.e1–e6, February 18, 2020 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER mouse: MyD88 / Adachi et al., 1998 n/a mouse: Tnf / Pasparakis et al., 1996 n/a mouse: Tnfr1 / Pfeffer et al., 1993 n/a mouse: Ifnar / M€uller et al., 1994 n/a Oligonucleotides qPCR primers see Table S1 n/a n/a Software and Algorithms ZEN (blue edition) Zeiss https://www.zeiss.com/microscopy/int/products/ microscope-software/zen.html FlowJo.10 n/a https://www.flowjo.com/ GraphPad Prism 8 n/a https://www.graphpad.com/ Fluostar Omega BMG Labtech https://www.bmglabtech.com/fluostar-omega/ Other Axioscan Zeiss n/a BDSymphony BD Biosciences n/a LightCycler 480 II Roche n/a Amersham Imager 600 GE Healthcare n/a
Protease Inhibitor:Article Title: OTULIN Prevents Liver Inflammation and Hepatocellular Carcinoma by Inhibiting FADD- and RIPK1 Kinase-Mediated Hepatocyte Apoptosis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER CK19 Lammer lab n/a hoip Damgaard lab n/a hoil-1 Walczak lab n/a Chemicals, Peptides, and Recombinant Proteins TRIzol reagent Thermo Fisher Scientific cat#15596026 mTNF This paper n/a Collagenase A Sigma-Aldrich cat#11088793001 GST-UBAN Ivan Dikic lab n/a DNase Roche cat#04 536 282 001 DMEM GIBCO cat# 41965-062 DMEM F12 GIBCO cat# 31330-038 non essential aminoacids Lonza cat#BE13-114E L-glutamin Lonza cat# BE17-605F bovine collagen 1 Nutacon n/a Sodium pyruvate Sigma-Aldrich cat# S-8636 Penicillin-Streptomycin GIBCO cat#15140-122 fetal calf serum Bodinco n/a Complete, EDTA-free Protease Inhibitor Cocktail Tablets Roche cat#11873580001 PhosStop Roche cat# 04906837001 Antigen retrieval buffer Dako cat# S-203130 hydrogen peroxide 30% Sigma-Aldrich cat# H-1009 Hematoxylin Mayer’s Sigma cat# 51275-500ml Entellan Merck cat# 107961 Neg 50 Kryo-Medium Thermo Fisher Scientific cat# 6506 Direct Red 80 Sigma cat# 365548 Ac-DEVD-MCA PeptaNova cat# 3171-V Critical Commercial Assays Aurum Total RNA Isolation Mini Kit BioRad cat# 7326820 Sensifast cDNA Synthesis Kit Bioline cat#BIO-65054 SensiFAST SYBR No-ROX Kit GeC Biotech cat# CSA-01190 Vectastain ELITE ABC Kit Standard Vector Laboratories cat# PK-6100 ImmPACT DAB Peroxidase (HRP) Substrate Vector Laboratories cat# SK-4105 Western Lightning Plus ECL Perkin Elmer cat# NEL101001EA TUNEL Millipore cat#17-141 Trygliceride colorimetric assay kit Cayman chemical cat#10010303 Deposited Data TCGA database n/a https://www.cancer.gov/about-nci/organization/ ccg/research/structural-genomics/tcga Experimental Models: Cell Lines mouse: primary hepatocytes this paper n/a OTULINKO MEFs this paper n/a Experimental Models: Organisms/Strains mouse: OTULINFL this paper n/a mouse: Alfp Cre Kellendonk et al., 2000 n/a mouse: MlklFL Murphy et al., 2013 n/a mouse: FaddFL Mc Guire al., 2010 n/a mouse: Ripk1D138N Polykratis et al., 2014 n/a (Continued on next page) Cell Reports 30, 2237–2247.e1–e6, February 18, 2020 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER mouse: MyD88 / Adachi et al., 1998 n/a mouse: Tnf / Pasparakis et al., 1996 n/a mouse: Tnfr1 / Pfeffer et al., 1993 n/a mouse: Ifnar / M€uller et al., 1994 n/a Oligonucleotides qPCR primers see Table S1 n/a n/a Software and Algorithms ZEN (blue edition) Zeiss https://www.zeiss.com/microscopy/int/products/ microscope-software/zen.html FlowJo.10 n/a https://www.flowjo.com/ GraphPad Prism 8 n/a https://www.graphpad.com/ Fluostar Omega BMG Labtech https://www.bmglabtech.com/fluostar-omega/ Other Axioscan Zeiss n/a BDSymphony BD Biosciences n/a LightCycler 480 II Roche n/a Amersham Imager 600 GE Healthcare n/a
Isolation:Article Title: OTULIN Prevents Liver Inflammation and Hepatocellular Carcinoma by Inhibiting FADD- and RIPK1 Kinase-Mediated Hepatocyte Apoptosis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER CK19 Lammer lab n/a hoip Damgaard lab n/a hoil-1 Walczak lab n/a Chemicals, Peptides, and Recombinant Proteins TRIzol reagent Thermo Fisher Scientific cat#15596026 mTNF This paper n/a Collagenase A Sigma-Aldrich cat#11088793001 GST-UBAN Ivan Dikic lab n/a DNase Roche cat#04 536 282 001 DMEM GIBCO cat# 41965-062 DMEM F12 GIBCO cat# 31330-038 non essential aminoacids Lonza cat#BE13-114E L-glutamin Lonza cat# BE17-605F bovine collagen 1 Nutacon n/a Sodium pyruvate Sigma-Aldrich cat# S-8636 Penicillin-Streptomycin GIBCO cat#15140-122 fetal calf serum Bodinco n/a Complete, EDTA-free Protease Inhibitor Cocktail Tablets Roche cat#11873580001 PhosStop Roche cat# 04906837001 Antigen retrieval buffer Dako cat# S-203130 hydrogen peroxide 30% Sigma-Aldrich cat# H-1009 Hematoxylin Mayer’s Sigma cat# 51275-500ml Entellan Merck cat# 107961 Neg 50 Kryo-Medium Thermo Fisher Scientific cat# 6506 Direct Red 80 Sigma cat# 365548 Ac-DEVD-MCA PeptaNova cat# 3171-V Critical Commercial Assays Aurum Total RNA Isolation Mini Kit BioRad cat# 7326820 Sensifast cDNA Synthesis Kit Bioline cat#BIO-65054 SensiFAST SYBR No-ROX Kit GeC Biotech cat# CSA-01190 Vectastain ELITE ABC Kit Standard Vector Laboratories cat# PK-6100 ImmPACT DAB Peroxidase (HRP) Substrate Vector Laboratories cat# SK-4105 Western Lightning Plus ECL Perkin Elmer cat# NEL101001EA TUNEL Millipore cat#17-141 Trygliceride colorimetric assay kit Cayman chemical cat#10010303 Deposited Data TCGA database n/a https://www.cancer.gov/about-nci/organization/ ccg/research/structural-genomics/tcga Experimental Models: Cell Lines mouse: primary hepatocytes this paper n/a OTULINKO MEFs this paper n/a Experimental Models: Organisms/Strains mouse: OTULINFL this paper n/a mouse: Alfp Cre Kellendonk et al., 2000 n/a mouse: MlklFL Murphy et al., 2013 n/a mouse: FaddFL Mc Guire al., 2010 n/a mouse: Ripk1D138N Polykratis et al., 2014 n/a (Continued on next page) Cell Reports 30, 2237–2247.e1–e6, February 18, 2020 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER mouse: MyD88 / Adachi et al., 1998 n/a mouse: Tnf / Pasparakis et al., 1996 n/a mouse: Tnfr1 / Pfeffer et al., 1993 n/a mouse: Ifnar / M€uller et al., 1994 n/a Oligonucleotides qPCR primers see Table S1 n/a n/a Software and Algorithms ZEN (blue edition) Zeiss https://www.zeiss.com/microscopy/int/products/ microscope-software/zen.html FlowJo.10 n/a https://www.flowjo.com/ GraphPad Prism 8 n/a https://www.graphpad.com/ Fluostar Omega BMG Labtech https://www.bmglabtech.com/fluostar-omega/ Other Axioscan Zeiss n/a BDSymphony BD Biosciences n/a LightCycler 480 II Roche n/a Amersham Imager 600 GE Healthcare n/a
cDNA Synthesis:Article Title: OTULIN Prevents Liver Inflammation and Hepatocellular Carcinoma by Inhibiting FADD- and RIPK1 Kinase-Mediated Hepatocyte Apoptosis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER CK19 Lammer lab n/a hoip Damgaard lab n/a hoil-1 Walczak lab n/a Chemicals, Peptides, and Recombinant Proteins TRIzol reagent Thermo Fisher Scientific cat#15596026 mTNF This paper n/a Collagenase A Sigma-Aldrich cat#11088793001 GST-UBAN Ivan Dikic lab n/a DNase Roche cat#04 536 282 001 DMEM GIBCO cat# 41965-062 DMEM F12 GIBCO cat# 31330-038 non essential aminoacids Lonza cat#BE13-114E L-glutamin Lonza cat# BE17-605F bovine collagen 1 Nutacon n/a Sodium pyruvate Sigma-Aldrich cat# S-8636 Penicillin-Streptomycin GIBCO cat#15140-122 fetal calf serum Bodinco n/a Complete, EDTA-free Protease Inhibitor Cocktail Tablets Roche cat#11873580001 PhosStop Roche cat# 04906837001 Antigen retrieval buffer Dako cat# S-203130 hydrogen peroxide 30% Sigma-Aldrich cat# H-1009 Hematoxylin Mayer’s Sigma cat# 51275-500ml Entellan Merck cat# 107961 Neg 50 Kryo-Medium Thermo Fisher Scientific cat# 6506 Direct Red 80 Sigma cat# 365548 Ac-DEVD-MCA PeptaNova cat# 3171-V Critical Commercial Assays Aurum Total RNA Isolation Mini Kit BioRad cat# 7326820 Sensifast cDNA Synthesis Kit Bioline cat#BIO-65054 SensiFAST SYBR No-ROX Kit GeC Biotech cat# CSA-01190 Vectastain ELITE ABC Kit Standard Vector Laboratories cat# PK-6100 ImmPACT DAB Peroxidase (HRP) Substrate Vector Laboratories cat# SK-4105 Western Lightning Plus ECL Perkin Elmer cat# NEL101001EA TUNEL Millipore cat#17-141 Trygliceride colorimetric assay kit Cayman chemical cat#10010303 Deposited Data TCGA database n/a https://www.cancer.gov/about-nci/organization/ ccg/research/structural-genomics/tcga Experimental Models: Cell Lines mouse: primary hepatocytes this paper n/a OTULINKO MEFs this paper n/a Experimental Models: Organisms/Strains mouse: OTULINFL this paper n/a mouse: Alfp Cre Kellendonk et al., 2000 n/a mouse: MlklFL Murphy et al., 2013 n/a mouse: FaddFL Mc Guire al., 2010 n/a mouse: Ripk1D138N Polykratis et al., 2014 n/a (Continued on next page) Cell Reports 30, 2237–2247.e1–e6, February 18, 2020 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER mouse: MyD88 / Adachi et al., 1998 n/a mouse: Tnf / Pasparakis et al., 1996 n/a mouse: Tnfr1 / Pfeffer et al., 1993 n/a mouse: Ifnar / M€uller et al., 1994 n/a Oligonucleotides qPCR primers see Table S1 n/a n/a Software and Algorithms ZEN (blue edition) Zeiss https://www.zeiss.com/microscopy/int/products/ microscope-software/zen.html FlowJo.10 n/a https://www.flowjo.com/ GraphPad Prism 8 n/a https://www.graphpad.com/ Fluostar Omega BMG Labtech https://www.bmglabtech.com/fluostar-omega/ Other Axioscan Zeiss n/a BDSymphony BD Biosciences n/a LightCycler 480 II Roche n/a Amersham Imager 600 GE Healthcare n/a
Western Blot:Article Title: OTULIN Prevents Liver Inflammation and Hepatocellular Carcinoma by Inhibiting FADD- and RIPK1 Kinase-Mediated Hepatocyte Apoptosis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER CK19 Lammer lab n/a hoip Damgaard lab n/a hoil-1 Walczak lab n/a Chemicals, Peptides, and Recombinant Proteins TRIzol reagent Thermo Fisher Scientific cat#15596026 mTNF This paper n/a Collagenase A Sigma-Aldrich cat#11088793001 GST-UBAN Ivan Dikic lab n/a DNase Roche cat#04 536 282 001 DMEM GIBCO cat# 41965-062 DMEM F12 GIBCO cat# 31330-038 non essential aminoacids Lonza cat#BE13-114E L-glutamin Lonza cat# BE17-605F bovine collagen 1 Nutacon n/a Sodium pyruvate Sigma-Aldrich cat# S-8636 Penicillin-Streptomycin GIBCO cat#15140-122 fetal calf serum Bodinco n/a Complete, EDTA-free Protease Inhibitor Cocktail Tablets Roche cat#11873580001 PhosStop Roche cat# 04906837001 Antigen retrieval buffer Dako cat# S-203130 hydrogen peroxide 30% Sigma-Aldrich cat# H-1009 Hematoxylin Mayer’s Sigma cat# 51275-500ml Entellan Merck cat# 107961 Neg 50 Kryo-Medium Thermo Fisher Scientific cat# 6506 Direct Red 80 Sigma cat# 365548 Ac-DEVD-MCA PeptaNova cat# 3171-V Critical Commercial Assays Aurum Total RNA Isolation Mini Kit BioRad cat# 7326820 Sensifast cDNA Synthesis Kit Bioline cat#BIO-65054 SensiFAST SYBR No-ROX Kit GeC Biotech cat# CSA-01190 Vectastain ELITE ABC Kit Standard Vector Laboratories cat# PK-6100 ImmPACT DAB Peroxidase (HRP) Substrate Vector Laboratories cat# SK-4105 Western Lightning Plus ECL Perkin Elmer cat# NEL101001EA TUNEL Millipore cat#17-141 Trygliceride colorimetric assay kit Cayman chemical cat#10010303 Deposited Data TCGA database n/a https://www.cancer.gov/about-nci/organization/ ccg/research/structural-genomics/tcga Experimental Models: Cell Lines mouse: primary hepatocytes this paper n/a OTULINKO MEFs this paper n/a Experimental Models: Organisms/Strains mouse: OTULINFL this paper n/a mouse: Alfp Cre Kellendonk et al., 2000 n/a mouse: MlklFL Murphy et al., 2013 n/a mouse: FaddFL Mc Guire al., 2010 n/a mouse: Ripk1D138N Polykratis et al., 2014 n/a (Continued on next page) Cell Reports 30, 2237–2247.e1–e6, February 18, 2020 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER mouse: MyD88 / Adachi et al., 1998 n/a mouse: Tnf / Pasparakis et al., 1996 n/a mouse: Tnfr1 / Pfeffer et al., 1993 n/a mouse: Ifnar / M€uller et al., 1994 n/a Oligonucleotides qPCR primers see Table S1 n/a n/a Software and Algorithms ZEN (blue edition) Zeiss https://www.zeiss.com/microscopy/int/products/ microscope-software/zen.html FlowJo.10 n/a https://www.flowjo.com/ GraphPad Prism 8 n/a https://www.graphpad.com/ Fluostar Omega BMG Labtech https://www.bmglabtech.com/fluostar-omega/ Other Axioscan Zeiss n/a BDSymphony BD Biosciences n/a LightCycler 480 II Roche n/a Amersham Imager 600 GE Healthcare n/a
TUNEL Assay:Article Title: OTULIN Prevents Liver Inflammation and Hepatocellular Carcinoma by Inhibiting FADD- and RIPK1 Kinase-Mediated Hepatocyte Apoptosis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER CK19 Lammer lab n/a hoip Damgaard lab n/a hoil-1 Walczak lab n/a Chemicals, Peptides, and Recombinant Proteins TRIzol reagent Thermo Fisher Scientific cat#15596026 mTNF This paper n/a Collagenase A Sigma-Aldrich cat#11088793001 GST-UBAN Ivan Dikic lab n/a DNase Roche cat#04 536 282 001 DMEM GIBCO cat# 41965-062 DMEM F12 GIBCO cat# 31330-038 non essential aminoacids Lonza cat#BE13-114E L-glutamin Lonza cat# BE17-605F bovine collagen 1 Nutacon n/a Sodium pyruvate Sigma-Aldrich cat# S-8636 Penicillin-Streptomycin GIBCO cat#15140-122 fetal calf serum Bodinco n/a Complete, EDTA-free Protease Inhibitor Cocktail Tablets Roche cat#11873580001 PhosStop Roche cat# 04906837001 Antigen retrieval buffer Dako cat# S-203130 hydrogen peroxide 30% Sigma-Aldrich cat# H-1009 Hematoxylin Mayer’s Sigma cat# 51275-500ml Entellan Merck cat# 107961 Neg 50 Kryo-Medium Thermo Fisher Scientific cat# 6506 Direct Red 80 Sigma cat# 365548 Ac-DEVD-MCA PeptaNova cat# 3171-V Critical Commercial Assays Aurum Total RNA Isolation Mini Kit BioRad cat# 7326820 Sensifast cDNA Synthesis Kit Bioline cat#BIO-65054 SensiFAST SYBR No-ROX Kit GeC Biotech cat# CSA-01190 Vectastain ELITE ABC Kit Standard Vector Laboratories cat# PK-6100 ImmPACT DAB Peroxidase (HRP) Substrate Vector Laboratories cat# SK-4105 Western Lightning Plus ECL Perkin Elmer cat# NEL101001EA TUNEL Millipore cat#17-141 Trygliceride colorimetric assay kit Cayman chemical cat#10010303 Deposited Data TCGA database n/a https://www.cancer.gov/about-nci/organization/ ccg/research/structural-genomics/tcga Experimental Models: Cell Lines mouse: primary hepatocytes this paper n/a OTULINKO MEFs this paper n/a Experimental Models: Organisms/Strains mouse: OTULINFL this paper n/a mouse: Alfp Cre Kellendonk et al., 2000 n/a mouse: MlklFL Murphy et al., 2013 n/a mouse: FaddFL Mc Guire al., 2010 n/a mouse: Ripk1D138N Polykratis et al., 2014 n/a (Continued on next page) Cell Reports 30, 2237–2247.e1–e6, February 18, 2020 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER mouse: MyD88 / Adachi et al., 1998 n/a mouse: Tnf / Pasparakis et al., 1996 n/a mouse: Tnfr1 / Pfeffer et al., 1993 n/a mouse: Ifnar / M€uller et al., 1994 n/a Oligonucleotides qPCR primers see Table S1 n/a n/a Software and Algorithms ZEN (blue edition) Zeiss https://www.zeiss.com/microscopy/int/products/ microscope-software/zen.html FlowJo.10 n/a https://www.flowjo.com/ GraphPad Prism 8 n/a https://www.graphpad.com/ Fluostar Omega BMG Labtech https://www.bmglabtech.com/fluostar-omega/ Other Axioscan Zeiss n/a BDSymphony BD Biosciences n/a LightCycler 480 II Roche n/a Amersham Imager 600 GE Healthcare n/a
Colorimetric Assay:Article Title: OTULIN Prevents Liver Inflammation and Hepatocellular Carcinoma by Inhibiting FADD- and RIPK1 Kinase-Mediated Hepatocyte Apoptosis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER CK19 Lammer lab n/a hoip Damgaard lab n/a hoil-1 Walczak lab n/a Chemicals, Peptides, and Recombinant Proteins TRIzol reagent Thermo Fisher Scientific cat#15596026 mTNF This paper n/a Collagenase A Sigma-Aldrich cat#11088793001 GST-UBAN Ivan Dikic lab n/a DNase Roche cat#04 536 282 001 DMEM GIBCO cat# 41965-062 DMEM F12 GIBCO cat# 31330-038 non essential aminoacids Lonza cat#BE13-114E L-glutamin Lonza cat# BE17-605F bovine collagen 1 Nutacon n/a Sodium pyruvate Sigma-Aldrich cat# S-8636 Penicillin-Streptomycin GIBCO cat#15140-122 fetal calf serum Bodinco n/a Complete, EDTA-free Protease Inhibitor Cocktail Tablets Roche cat#11873580001 PhosStop Roche cat# 04906837001 Antigen retrieval buffer Dako cat# S-203130 hydrogen peroxide 30% Sigma-Aldrich cat# H-1009 Hematoxylin Mayer’s Sigma cat# 51275-500ml Entellan Merck cat# 107961 Neg 50 Kryo-Medium Thermo Fisher Scientific cat# 6506 Direct Red 80 Sigma cat# 365548 Ac-DEVD-MCA PeptaNova cat# 3171-V Critical Commercial Assays Aurum Total RNA Isolation Mini Kit BioRad cat# 7326820 Sensifast cDNA Synthesis Kit Bioline cat#BIO-65054 SensiFAST SYBR No-ROX Kit GeC Biotech cat# CSA-01190 Vectastain ELITE ABC Kit Standard Vector Laboratories cat# PK-6100 ImmPACT DAB Peroxidase (HRP) Substrate Vector Laboratories cat# SK-4105 Western Lightning Plus ECL Perkin Elmer cat# NEL101001EA TUNEL Millipore cat#17-141 Trygliceride colorimetric assay kit Cayman chemical cat#10010303 Deposited Data TCGA database n/a https://www.cancer.gov/about-nci/organization/ ccg/research/structural-genomics/tcga Experimental Models: Cell Lines mouse: primary hepatocytes this paper n/a OTULINKO MEFs this paper n/a Experimental Models: Organisms/Strains mouse: OTULINFL this paper n/a mouse: Alfp Cre Kellendonk et al., 2000 n/a mouse: MlklFL Murphy et al., 2013 n/a mouse: FaddFL Mc Guire al., 2010 n/a mouse: Ripk1D138N Polykratis et al., 2014 n/a (Continued on next page) Cell Reports 30, 2237–2247.e1–e6, February 18, 2020 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER mouse: MyD88 / Adachi et al., 1998 n/a mouse: Tnf / Pasparakis et al., 1996 n/a mouse: Tnfr1 / Pfeffer et al., 1993 n/a mouse: Ifnar / M€uller et al., 1994 n/a Oligonucleotides qPCR primers see Table S1 n/a n/a Software and Algorithms ZEN (blue edition) Zeiss https://www.zeiss.com/microscopy/int/products/ microscope-software/zen.html FlowJo.10 n/a https://www.flowjo.com/ GraphPad Prism 8 n/a https://www.graphpad.com/ Fluostar Omega BMG Labtech https://www.bmglabtech.com/fluostar-omega/ Other Axioscan Zeiss n/a BDSymphony BD Biosciences n/a LightCycler 480 II Roche n/a Amersham Imager 600 GE Healthcare n/a
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